Immunohistochemistry analysis of mouse inflamed colon, fixed in formalin. The Primary Antibody used was Anti-HSC70 Antibody (A304779) at 1:50,000 for 12 hours at 4°C. The secondary antibody used was Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at room temperature. Counterstain: Methyl Green at 200uL for 2 min at room temperature.
Western blot analysis of human Cell line lysates showing detection of HSC70 protein using Anti-HSC70 Antibody (A304779) at 1:1,000 for 2 hours at room temperature. Load: 15 µgprotein. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Donkey Anti-Rabbit IgG: HRP for 1 hour at room temperature.
Immunohistochemistry analysis of human colon carcinoma, fixed in formalin. The Primary Antibody used was Anti-HSC70 Antibody (A304779) at 1:10,000 for 12 hours at 4°C. The secondary antibody used was Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at room temperature. Counterstain: Methyl Green at 200uL for 2 min at room temperature.
Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution. The Primary Antibody used was Anti-HSC70 Antibody (A304779) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at room temperature. Localization: Cytoplasm.
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