Anti-HP1 alpha Antibody (A82447)

HP1 alpha expression in A43N (A) and K562 (B) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-HP1 alpha Antibody (A82447) at 0.03µg/ml and detected by chemiluminescence.

HP1 alpha expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-HP1 alpha Antibody (A82447) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 4µg/ml. Nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 4µg/ml.

HP1 alpha expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-HP1 alpha Antibody (A82447) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.