Anti-HP1 alpha Antibody (A13174)

Name: Anti-HP1 alpha Antibody
Brand: Antibodies.com
SKU: A13174
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to HP1 alpha for WB, IHC-P, ICC/IF, IP, ChIP and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Tags:

Heterochromatin Marker

Specifications

Name

Anti-HP1 alpha Antibody

Description

Rabbit polyclonal antibody to HP1 alpha.

Applications

WB, IHC-P, ICC/IF, IP, ChIP, ELISA

Positive Control

HeLa, MCF7, A-549, SH-SY5Y.

Dilutions

WB: 1:500-1:2,000, IHC-P: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 0.5µg-4µg antibody for 200µg-400µg extracts of whole cells, ELISA: 1 µg/ml (starting concentration), ChIP: 1:20-1:100

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant fusion protein corresponding to amino acids 1-191 of human HP1 alpha.

Sequence

MGKKTKRTADSSSSEDEEEYVVEKVLDRRVVKGQVEYLLKWKGFSEEHNTWEPEKNLDCPELISEFMKKYKKMKEGENNKPREKSESNKRKSNFSNSADDIKSKKKREQSNDIARGFERGLEPEKIIGATDSCGDLMFLMKWKDTDEADLVLAKEANVKCPQIVIAFYEERLTWHAYPEDAENKEKETAKS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

22 kDa

Predicted MW

22 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Antigen p25, CBX5, Chromobox protein homolog 5, Heterochromatin protein 1 homolog alpha, HP1A

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

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Western Blot - Anti-HP1 alpha Antibody (A13174)

HP1 alpha expression in various lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-HP1 alpha Antibody (A13174) at a dilution of 1:1000 and detected with chemiluminescence.

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Immunohistochemistry - Anti-HP1 alpha Antibody (A13174)

HP1 alpha expression in rat hippocampus analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by microwaving in 10 mM PBS buffer, pH 7.2. Staining was performed with Anti-HP1 alpha Antibody (A13174) at a dilution of 1:100.

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Immunohistochemistry - Anti-HP1 alpha Antibody (A13174)

HP1 alpha expression in human brain astrocytoma tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by microwaving in 10 mM PBS buffer, pH 7.2. Staining was performed with Anti-HP1 alpha Antibody (A13174) at a dilution of 1:100.

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Immunohistochemistry - Anti-HP1 alpha Antibody (A13174)

HP1 alpha expression in mouse kidney tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by microwaving in 10 mM PBS buffer, pH 7.2. Staining was performed with Anti-HP1 alpha Antibody (A13174) at a dilution of 1:100.