Rabbit polyclonal antibody to hnRPD (Phospho-Ser83)
Specificity
The antibody detects endogenous levels of hnRPD only when phosphorylated at serine 83.
Applications
WB, IHC, IF
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200
Reactivity
Human, Mouse
Immunogen
Peptide sequence around phosphorylation site of Serine 83(N-S-S(p)-P-R) derived from Human hnRPD .
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C / 1 year
Synonyms
AU-rich element RNA-binding protein 1, AUF1, Heterogeneous nuclear ribonucleoprotein D0, hnRNP D0, HNRNPD
Western blot analysis of extracts from JK cells (Lane 2), using hnRPD (Phospho-Ser83) Antibody #11696. The lane on the left is treated with antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using hnRPD (Phospho-Ser83) antibody #11696 (left)or the same antibody preincubated with blocking peptide (right).