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Anti-hnRNP U/p120 Antibody (A385)

Immunoblotting of hnRNP-U with this antibody (ref.2). Specificity of anti-hnRNP-U antibody, HUT. Cell lysates were prepared from SAOS-2 cells transfected with pRc/CMV vectors (pRc) or pRc/CMV vectors expressing Myc-tagged hnRNP-U (Myc-UF). Exogenous Myc-tagged hnRNP-U (Myc-U) and endogenous hnRNP-U (U) proteins were detected by immunoblotting with anti-Myc antibody (aMyc) or HUT (aU). This antibody recognized exogenous Myc-tagged hnRNP-U and endogenous ~120 kDa hnRNP-U proteins in SAOS-2 cells. Immunocytochemistry using this antibody, HUT (ref.2) Mouse P19 neurons were labeled with anti-necdin antibody (Ndn) (a) or with HUT for hnRNP-U (U) (c) in combination with anti-neuronal marker, MAP2, antibody for MAP2 (b, d). The nuclear matrix was prepared in situ and labeled for necdin (Ndn) (e), hnRNP-U (U) (f), and a nuclear matrix marker, lamin B (g). Both necdin and hnRNP-U were localized to the nuclei of differentiated neurons, which express the neuronal marker MAP2 (a-d). Necdin was also distributed in the neuronal cytoplasm (a). The immunocytochemical analysis of in situ extracted nuclear matrix revealed that both necdin and hnRNP-U were concentrated in intranuclear speckles throughout the nucleoplasm (e, f). Lamin B, a nuclear matrix marker, was localized to the nuclear lamina (g). These results suggest that both necdin and hnRNP-U are associated with the nuclear matrix of neurons
$530
Product Datasheet

Rabbit polyclonal antibody to hnRNP U/p120 for WB, IP and ICC.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.

  • Specifications

Name
Anti-hnRNP U/p120 Antibody
Description
Rabbit polyclonal antibody to hnRNP U/p120.
Applications
WB, IP, ICC
Dilutions
WB: 1:1,000-1:3,000, ICC: 1:500-1:1,000
Reactivity
Mouse, Rat
Immunogen
Synthetic peptide corresponding to mouse hnRNP-U protein (a.a. 614-800), fused to MBT.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Product Form
Liquid
Formulation
Supplied as antiserum with 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Immunoblotting of hnRNP-U with this antibody (ref.2). Specificity of anti-hnRNP-U antibody, HUT. Cell lysates were prepared from SAOS-2 cells transfected with pRc/CMV vectors (pRc) or pRc/CMV vectors expressing Myc-tagged hnRNP-U (Myc-UF). Exogenous Myc-tagged hnRNP-U (Myc-U) and endogenous hnRNP-U (U) proteins were detected by immunoblotting with anti-Myc antibody (aMyc) or HUT (aU). This antibody recognized exogenous Myc-tagged hnRNP-U and endogenous ~120 kDa hnRNP-U proteins in SAOS-2 cells.Enlarge Image

Anti-hnRNP U/p120 Antibody (A385)

Anti-hnRNP U/p120 Antibody
Immunocytochemistry using this antibody, HUT (ref.2) Mouse P19 neurons were labeled with anti-necdin antibody (Ndn) (a) or with HUT for hnRNP-U (U) (c) in combination with anti-neuronal marker, MAP2, antibody for MAP2 (b, d). The nuclear matrix was prepared in situ and labeled for necdin (Ndn) (e), hnRNP-U (U) (f), and a nuclear matrix marker, lamin B (g). Both necdin and hnRNP-U were localized to the nuclei of differentiated neurons, which express the neuronal marker MAP2 (a-d). Necdin was also distributed in the neuronal cytoplasm (a). The immunocytochemical analysis of in situ extracted nuclear matrix revealed that both necdin and hnRNP-U were concentrated in intranuclear speckles throughout the nucleoplasm (e, f). Lamin B, a nuclear matrix marker, was localized to the nuclear lamina (g). These results suggest that both necdin and hnRNP-U are associated with the nuclear matrix of neuronsEnlarge Image

Immunocytochemistry - Anti-hnRNP U/p120 Antibody (A385)

Anti-hnRNP U/p120 Antibody
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