Hmox1 expression in NIH3T3 cell lysates analyzed by western blot. Cells were treated with arsenite (50 µM) at 37°C for 8 hours. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-Hmox1 Antibody (A329438) at a dilution of 1:1000 and detected with chemiluminescence.
Hmox1 expression in various lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-Hmox1 Antibody (A329438) at a dilution of 1:1000 and detected with chemiluminescence.
Hmox1 expression in rat spleen tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved with 10 mM citrate buffer, pH 6.0, under high pressure. Staining was performed with Anti-Hmox1 Antibody (A329438) at a dilution of 1:200.
Hmox1 expression in Rat spleen tissue analyzed by immunofluorescence. Staining was performed with Anti-Hmox1 Antibody (A329438) at a dilution of 1:100 followed by Cy3-conjugated Goat anti-Rabbit IgG (H+L)secondary antibody at a dilution of 1:500. Nuclei were stained with DAPI (blue).
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