Anti-HMGB1 Antibody [HMG1-5H6] (A280631)

Anti-HMGB1 Antibody [HMG1-5H6]

Hamster monoclonal [HMG1-5H6] antibody to HMGB1.

This antibody recognises high mobility group protein B1 (HMGB1), a ubiquitously expressed nuclear DNA binding protein belonging to the alarmin family. HMGB1 is released by necrotic and inflammatory cells acting as a chemoattractant for immature dendritic cells (DCs) and promoting their maturation. DCs can also secrete HMGB1, leading to proliferation and Th1 polarization of interacting T cells. HMGB1 is angiogenic and promotes cardiac stem cell growth and differentiation, it is a damage-associated molecular pattern molecule (DAMP) which co-precipitates with CD24.

WB, IF, Flow Cytometry

Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

Mouse, Rat, Human, Hamster

Recombinant mouse HMGB1.

Hamster

Monoclonal

HMG1-5H6

IgG

1 mg/ml

Approximately 30 kDa in mouse NIH-3T3, rat NRK, hamster BHK, and HeLa cell lysates in the presence of 2ME, and bands of 30 kDa (red) and 27 kDa (ox) in the absence of 2ME.

Liquid

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Hamster anti High Mobility Group Protein B1 antibody, clone HMG1-5H6 recognizes high mobility group protein B1 (HMGB1), a ubiquitously expressed nuclear DNA binding protein belonging to the alarmin family.HMGB1 is released by necrotic and inflammatory cells acting as a chemoattractant for immature dendritic cells (DCs) and promoting their maturation. DCs can also secrete HMGB1, leading to proliferation and Th1 polarization of interacting T cells. HMGB1 is angiogenic and promotes cardiac stem cell growth and differentiation, it is a damage-associated molecular pattern molecule (DAMP) which co-precipitates with CD24.

High mobility group protein 1, High mobility group protein B1, HMG-1, HMG1