Full-length recombinant human HMGB1 protein, expressed in and purified from E. coli.
Host
Mouse
Clonality
Monoclonal
Clone ID
1F3
Isotype
IgG2b
Conjugate
Unconjugated
Purification
Immunogen affinity purification.
Concentration
1 mg/ml
Molecular Weight
25 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol and 5mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
General Notes
The HMGB1 protein is normally located in the nucleus where it associates with chromatin and DNA and, as such, this antibody is a useful nuclear marker.
Synonyms
High mobility group protein 1, High mobility group protein B1, HMG-1, HMG1
Immunofluorescent analysis of of HeLa cells stained with Anti-HMGB1 Antibody [1F3] (A85355), dilution 1:2,000, in red and co-stained with Anti-GAPDH Antibody (A85377), dilution 1:2,000 in green. Anti-HMGB1 Antibody [1F3] stains the chromatin binding protein HMGB1, which is localized in the nuclei. In contrast the Anti-GAPDH Antibody produces strong cytoplasmic staining of cells.
Western blot analysis of lysates from different cell lines probed with Anti-HMGB1 Antibody [1F3] (A85355), dilution 1:2,000. The lanes contain: [1] protein standard, [2] NIH-3T3, [3] C6, [4] HEK293, [5] HeLa, and [6] SH-SY5Y cell lystates. The 25 kDa band revealed by Anti-HMGB1 Antibody [1F3] corresponds to the HMGB1 protein.
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![Immunofluorescence - Anti-HMGB1 Antibody [1F3] (A85355) - Antibodies.com](https://cdn.antibodies.com/image/catalog/85/A85355_1.jpg?profile=product_top)
![Western Blot - Anti-HMGB1 Antibody [1F3] (A85355) - Antibodies.com](https://cdn.antibodies.com/image/catalog/85/A85355_2.jpg?profile=product_top)
![Immunofluorescence - Anti-HMGB1 Antibody [1F3] (A85355) - Antibodies.com](https://cdn.antibodies.com/image/catalog/85/A85355_1.jpg?profile=product_top_thumb)
![Western Blot - Anti-HMGB1 Antibody [1F3] (A85355) - Antibodies.com](https://cdn.antibodies.com/image/catalog/85/A85355_2.jpg?profile=product_top_thumb)
![Immunofluorescence - Anti-HMGB1 Antibody [1F3] (A85355) - Antibodies.com](https://cdn.antibodies.com/image/catalog/85/A85355_1.jpg?profile=product_image)
![Western Blot - Anti-HMGB1 Antibody [1F3] (A85355) - Antibodies.com](https://cdn.antibodies.com/image/catalog/85/A85355_2.jpg?profile=product_image)
![Western Blot - Anti-HMGB1 Antibody [ARC0001] (A308594) - Antibodies.com](https://cdn.antibodies.com/image/catalog/308/A308594_1.jpg?profile=product_alternative)
