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Anti-HMGA1 Antibody [ARC1060] (A307749)

Western Blot - Anti-HMGA1 Antibody [ARC1060] (A307749) - Antibodies.com Western Blot - Anti-HMGA1 Antibody [ARC1060] (A307749) - Antibodies.com Immunohistochemistry - Anti-HMGA1 Antibody [ARC1060] (A307749) - Antibodies.com Immunohistochemistry - Anti-HMGA1 Antibody [ARC1060] (A307749) - Antibodies.com Immunofluorescence - Anti-HMGA1 Antibody [ARC1060] (A307749) - Antibodies.com Immunofluorescence - Anti-HMGA1 Antibody [ARC1060] (A307749) - Antibodies.com
$485
Product Datasheet

Rabbit monoclonal [ARC1060] antibody to HMGA1 for WB, IHC, ICC/IF, IP and ChIP.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-HMGA1 Antibody [ARC1060]
Description
Rabbit monoclonal [ARC1060] antibody to HMGA1.
Applications
WB, IHC, ICC/IF, IP, ChIP
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, ChIP: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-107 of human HMGA1 (P17096).
Sequence
MSESSSKSSQPLASKQEKDGTEKRGRGRPRKQPPVSPGTALVGSQKEPSEVPTPKRPRGRPKGSKNKGAAKTRKTTTTPGRKPRGRPKKLEKEEEEGISQESSEEEQ
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC1060
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
18 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
High mobility group AT-hook protein 1, High mobility group protein A1, High mobility group protein HMG-I/HMG-Y, High mobility group protein R, HMG-I(Y), HMGIY
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (6)

Western Blot - Anti-HMGA1 Antibody [ARC1060] (A307749) - Antibodies.comEnlarge Image

Western Blot - Anti-HMGA1 Antibody [ARC1060] (A307749)

Western blot analysis of extracts of various cell lines, using Anti-HMGA1 Antibody [ARC1060] (A307749) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Western Blot - Anti-HMGA1 Antibody [ARC1060] (A307749) - Antibodies.comEnlarge Image

Western Blot - Anti-HMGA1 Antibody [ARC1060] (A307749)

Western blot analysis of extracts of Mouse testis, using Anti-HMGA1 Antibody [ARC1060] (A307749) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry - Anti-HMGA1 Antibody [ARC1060] (A307749) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-HMGA1 Antibody [ARC1060] (A307749)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-HMGA1 Antibody [ARC1060] (A307749) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-HMGA1 Antibody [ARC1060] (A307749) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-HMGA1 Antibody [ARC1060] (A307749)

Immunohistochemistry analysis of paraffin-embedded human oophoroma using Anti-HMGA1 Antibody [ARC1060] (A307749) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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