This antibody reacts with native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. Reactivity with HLA-G3 was also reported.
Applications
Flow Cytometry, IP, IHC-Fr, ICC, ELISA
Dilutions
Flow Cytometry: 0.3-4 µg/ml; Positive Control: JEG-3 human choriocarcinoma cell line, IHC: 2-5 µg/ml, ELISA: This antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human HLA-G in combination with antibody B2M-01 or with antibody W6/32. Coating antibody 10 µg/ml, detection antibody (biotin or peroxidase conjugate) 1 µg/ml.
Reactivity
Human
Cross Reactivity
This antibody does not cross react with Mouse.
Immunogen
Recombinant human HLA-G refolded with beta2-microglobulin and peptide.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-G/9
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
HLA class I histocompatibility antigen, alpha chain G, HLA G antigen, HLA-6.0, HLAG, MHC class I antigen G
Western Blot - Anti-HLA G Antibody [MEM-G/9] (A86211)
Immunoprecipitation of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by Anti-HLA G Antibody (A86211) and protein G. HLA-G was detected by Anti-HLA-G Antibody [4H84] and Goat Anti-Mouse IgG Antibody (HRP) in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).
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