Anti-HLA G Antibody [87G] - Low endotoxin, Azide free
Description
Mouse monoclonal [87G] antibody to HLA G.
Specificity
This antibody recognizes both membrane-bound and soluble forms of HLA G (HLA G1 and HLA G5). HLA G belongs to the MHC Class I molecules and it is expressed on the surface of trophoblast cells. This antibody blocks interaction of HLA G with inhibitory receptors.
Applications
Flow Cytometry, IHC-Fr, ELISA
Dilutions
Flow Cytometry: Extracellular and Intracellular staining: 2 µg/ml; Positive Control: JEG-3 human choriocarcinoma epithelial cell line, IHC-Fr: 10 µg/ml, incubation: 20 min at 25°C; Positive Control: extravillous cytotrophoblast, ELISA: 1 µg/ml; Positive Control: JEG-3 human choriocarcinoma epithelial cell line. This antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human HLA-G in combination with antibody W6/32.
Reactivity
Human
Cross Reactivity
This antibody does not cross react with Mouse or Rat.
Immunogen
HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin.
Host
Mouse
Clonality
Monoclonal
Clone ID
87G
Isotype
IgG2a
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, without Sodium Azide (0.2 µm filter sterilized). Endotoxin level is less than 0.01 EU/µg of the protein.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
HLA class I histocompatibility antigen, alpha chain G, HLA G antigen, HLA-6.0, HLAG, MHC class I antigen G
Analysis of cytolytical activity of human polyclonal NK cells on target melanoma cells. Blocking of HLA-G1 on transfectants with Anti-human HLA-G (87G) restored specific lysis. Target cells: M8 cell line transfected with empty vector (Column 1) and with HLA-G1 cDNA (Columns 2-6). Blocking antibodies: Column 1-2: None. Column 3: Isotype mouse IgG2a control. Column 4: Anti-HLA-G Antibody [87G]. Column 5: Anti-HLA-G Antibody [87G] F(ab´)2 fragment. Column 6: Anti-HLA-G Antibody [MEM-G/9].
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