This antibody recognizes the membrane-bound form of HLA G (full-length HLA G1) but does not recognize soluble forms. HLA G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 5 µg/ml.
Reactivity
Human
Cross Reactivity
This antibody does not cross react with Mouse or Rat.
Immunogen
HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta 2 Microglobulin.
Host
Mouse
Clonality
Monoclonal
Clone ID
01G
Isotype
IgG1
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
This antibody is conjugated with Fluorescein Isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
HLA class I histocompatibility antigen, alpha chain G, HLA G antigen, HLA-6.0, HLAG, MHC class I antigen G