This antibody, also known as clone 3D12HLA-E, recognizes an extracellular part of HLA E, an ubiquitously expressed non-classical MHC class I molecule, as well as free HLA E.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1-5 µg/ml.
Reactivity
Human
Immunogen
Recombinant human HLA-E.
Host
Mouse
Clonality
Monoclonal
Clone ID
3D12
Isotype
IgG1
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
HLA class I histocompatibility antigen, alpha chain E, HLA-6.2, HLAE, MHC class I antigen E
Flow Cytometry - Anti-HLA E Antibody [3D12] (PE) (A121830)
Separation of human lymphocytes (red-filled) from blood debris (black-dashed) in flow cytometry analysis of human peripheral whole blood using Anti-HLA E Antibody [3D12] (PE). Concentration in sample is 2µg/ml.
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