This antibody specifically binds to the empty but not peptide-loaded form of HLA-DR1. DR is the isotypes of human MHC Class II molecules expressed on antigen-presenting cells (i.e. APC: dendritic cells, B lymphocytes, monocytes, macrophages).
Applications
WB, Flow Cytometry, ELISA
Dilutions
Flow Cytometry: Stains immature dendritic cells that express empty cell surface MHC molecules, but not cells that express predominantly peptide loaded forms.
Reactivity
Human
Immunogen
Purified, insoluble DR1 beta chain (DRB1*0101) expressed in E. coli inclusion bodies.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-267
Isotype
IgG2b
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
HLA class II histocompatibility antigen, DR alpha chain, HLA-DRA, HLA-DRA1, MHC class II antigen DRA