Anti-HLA Class I Antibody [MEM-147] (PE) (A85471)

Anti-HLA Class I Antibody [MEM-147] (PE)

Mouse monoclonal [MEM-147] antibody to HLA Class I (PE).

The antibody recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types. The antibody MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32.

Flow Cytometry: 1-2 µg/ml.

Human

PHA-activated peripheral blood lymphocytes.

Mouse

Monoclonal

IgG1

This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.

100 µg/ml

Liquid

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!

This product is for research use only. It is not intended for diagnostic or therapeutic use.