Mouse monoclonal [MEM-147] antibody to HLA Class I.
Specificity
The antibody recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human nucleated cell types. The antibody MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32.
Western Blot - Anti-HLA Class I Antibody [MEM-147] (A85472)
Western blot of human Ramos B cell line lysate (1% laurylmaltoside); non-reduced sample, immunostained by Anti-HLA Class I Antibody (A85472) and Goat Anti-Mouse IgG Antibody (H+L) (HRP).
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