This antibody recognises the human HLA-A2 histocompatability antigen. The epitope recognized by this antibody has been studied extensively and would appear to include the carboxy-terminus of the alpha-2 helix and a turn on one of the underlying beta strands. Mouse anti Human HLA A2 antibody, clone BB7.2 may be used for the flow cytometric detection of HLA-A2 expression and has also been used for immunoaffinity purification of HLA-A2 molecules. Functionally Mouse anti Human HLA A2 antibody, clone BB7.2 is reported to inhibit MHC restricted cellular cytotoxicity.
Applications
IP, Flow Cytometry, IHC-Fr
Dilutions
Flow Cytometry: 10 µg/ml, Use 10µl of the suggested working dilution to label 106 cells or 100µl whole blood
Reactivity
Human
Immunogen
Papain solubilized HLA-A2.
Host
Mouse
Clonality
Monoclonal
Clone ID
BB7.2
Isotype
IgG2b
Conjugate
Unconjugated
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Human HLA A2 antibody, clone BB7.2 recognizes the human HLA-A2 histocompatability antigen. The epitope recognized by this antibody has been studied extensively and would appear to include the carboxy-terminus of the alpha-2 helix and a turn on one of the underlying beta strands. Mouse anti Human HLA A2 antibody, clone BB7.2 may be used for the flow cytometric detection of HLA-A2 expression and has also been used for immunoaffinity purification of HLA-A2 molecules. Functionally Mouse anti Human HLA A2 antibody, clone BB7.2 is reported to inhibit MHC restricted cellular cytotoxicity.
Synonyms
HLA class I histocompatibility antigen, A alpha chain, HLA-A, HLAA, Human leukocyte antigen A