Anti-HLA-A Antibody [ARC0588] (A81042)

Name: Anti-HLA-A Antibody [ARC0588]
Brand: Antibodies.com
SKU: A81042
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC0588] antibody to HLA-A for WB, IHC and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-HLA-A Antibody [ARC0588]

Description

Rabbit monoclonal [ARC0588] antibody to HLA-A.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:1,000-1:5,000

Reactivity

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HLA-A (P04439).

Sequence

MAVMAPRTLLLLLSGALALTQTWAGSHSMRYFFTSVSRPGRGEPRFIAVGYVDDTQFVRFDSDAASQRMEPRAPWIEQEGPEYWDQETRNVKAQSQTDRV

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0588

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

41 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

HLA class I histocompatibility antigen, A alpha chain, HLAA, Human leukocyte antigen A

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-HLA-A Antibody [ARC0588] (A81042)

Western blot analysis of extracts of various cell lines, using Anti-HLA-A Antibody [ARC0588] (A81042) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Immunohistochemistry - Anti-HLA-A Antibody [ARC0588] (A81042)

Immunohistochemistry analysis of paraffin-embedded human esophageal using Anti-HLA-A Antibody [ARC0588] (A81042) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Western Blot - Anti-HLA-A Antibody [ARC0588] (A81042)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-HLA-A Antibody [ARC0588] (A81042). This Western blot was performed on the immunoprecipitate using Anti-HLA-A Antibody [ARC0588] (A81042) at a dilution of 1:2000.

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