Western Blot - Anti-Histone H4 (phospho Ser1) Antibody [RM194] (A121195)
Western blot of (1) acid extracts of HeLa cells, and (2) recombinant Histone H4, using Anti-Histone H4 (phospho Ser1) Antibody [RM194] at 0.5µg/ml, showed a band of Histone H4 phosphorylated at Serine 1 in HeLa cells.
Anti-Histone H4 (phospho Ser1) Antibody [RM194] specifically reacts to Histone H4 phosphorylated at Serine 1 (H4S1p). The reactivity is not affected by neighboring Arginine 3 modifications (H4S1pR3me1 and H4S1pR3me2). There is no cross reactivity with Histone H2A phosphorylated at Serine 1 (H2AS1p).
Immunocytochemistry of HeLa cells, using Anti-Histone H4 (phospho Ser1) Antibody [RM194] (red). Actin filaments have been labeled with fluorescein phalloidin (green).
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