Anti-Histone H4 (di methyl Arg3) Antibody (A16733)

Western blot analysis of various lysates, using Anti-Histone H4 (di methyl Arg3) Antibody (A16733) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

Dot blot analysis of a mixture of methylation peptides using Anti-Histone H4 (di methyl Arg3) Antibody (A16733).

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-Histone H4 (di methyl Arg3) Antibody (A16733) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of 293T cells using Anti-Histone H4 (di methyl Arg3) Antibody (A16733). DAPI was used to stain the cell nuclei (blue).