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Recombinant Anti-Histone H4 (acetyl Lys8) Antibody [RM201] (A121198)

Multiplex Immunoassay - Anti-Histone H4 (acetyl Lys8) Antibody [RM201] (A121198) - Antibodies.com Western Blot - Anti-Histone H4 (acetyl Lys8) Antibody [RM201] (A121198) - Antibodies.com Immunocytochemistry - Anti-Histone H4 (acetyl Lys8) Antibody [RM201] (A121199) - Antibodies.com
Recombinant Antibody
$585
Product Datasheet

Recombinant rabbit monoclonal [RM201] antibody to Histone H4 (acetyl Lys8) for WB, ELISA, Multiplex Protein Detection, ICC and ChIP.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.

  • Specifications

Name
Anti-Histone H4 (acetyl Lys8) Antibody [RM201]
Description
Recombinant rabbit monoclonal [RM201] antibody to Histone H4 (acetyl Lys8).
Specificity
This antibody recognizes Histone H4 acetylated at Lysine 8.
Applications
WB, ELISA, Multiplex Protein Detection, ICC, ChIP
Dilutions
WB: 0.5-2 µg/ml, ELISA: 0.2-1 µg/ml, Multiplex Protein Detection: 0.05-1 µg/ml
Reactivity
All Vertebrates
Cross Reactivity
There is no cross reactivity with other acetylated Lysines in Histone H4.
Immunogen
Synthetic peptide corresponding to Histone H4 (acetyl Lys8).
Host
Rabbit
Clonality
Monoclonal
Clone ID
RM201
Isotype
IgG
Conjugate

Unconjugated

Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
H4/A, H4C1, H4FA, HIST1H4A
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Multiplex Immunoassay - Anti-Histone H4 (acetyl Lys8) Antibody [RM201] (A121198) - Antibodies.comEnlarge Image

Anti-Histone H4 (acetyl Lys8) Antibody [RM201] (A121198)

Anti-Histone H4 (acetyl Lys8) Antibody [RM201] specifically reacts to Histone H4 acetylated at Lysine 8 (K8ac). There is no cross reactivity with unmodified Lysine 8 (K8 ctrl), acetylated Lysine 5 (K5ac), Lysine 12 (K12ac), Lysine 16 (K16ac), Lysine 20 (K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.
Western Blot - Anti-Histone H4 (acetyl Lys8) Antibody [RM201] (A121198) - Antibodies.comEnlarge Image

Western Blot - Anti-Histone H4 (acetyl Lys8) Antibody [RM201] (A121198)

Western blot of (1) acid extracts of HeLa cells treated with sodium butyrate, (2) acid extracts of HeLa cells untreated, and (3) recombinant Histone H4, using Anti-Histone H4 (acetyl Lys8) Antibody [RM201] at 0.5µg/ml, showed a band of Histone H4 acetylated at Lysine 8 in HeLa cells.
Immunocytochemistry - Anti-Histone H4 (acetyl Lys8) Antibody [RM201] (A121199) - Antibodies.comEnlarge Image

Immunocytochemistry - Anti-Histone H4 (acetyl Lys8) Antibody [RM201] (A121198)

Immunocytochemical staining of HeLa cells treated with sodium butyrate, using Anti-Histone H4 (acetyl Lys8) Antibody [RM201] (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Publishing research using Recombinant Anti-Histone H4 (acetyl Lys8) Antibody [RM201] (A121198)? Please let us know so that we can list the citation on this page.

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