Anti-Histone H4 (acetyl Lys8) Antibody (A16744)

Western blot analysis of extracts of various cell lines, using Anti-Histone H4 (acetyl Lys8) Antibody (A16744) at 1:1,000 dilution. NIH/3T3 cells and C6 cells were treated by TSA (1 uM) at 37°C for 18 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry analysis of paraffin-embedded human kidney using Anti-Histone H4 (acetyl Lys8) Antibody (A16744) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-Histone H4 (acetyl Lys8) Antibody (A16744) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Histone H4 (acetyl Lys8) Antibody (A16744) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.