Recombinant Anti-Histone H4 (acetyl Lys5) Antibody [RM199] (A121199)

Anti-Histone H4 (acetyl Lys5) Antibody [RM199] specifically reacts to Histone H4 acetylated at Lysine 5 (K5ac). There is no cross reactivity with unmodified Lysine 5 (K5 ctrl), acetylated Lysine 8 (K8ac), Lysine 12 (K12ac), Lysine 16 (K16ac), Lysine 20 (K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.

Western blot of (1) acid extracts of HeLa cells, and (2) recombinant Histone H4, using Anti-Histone H4 (acetyl Lys5) Antibody [RM199] at 0.2µg/ml, showed a band of Histone H4 acetylated at Lysine 5 in HeLa cells.

Immunocytochemical staining of HeLa cells treated with sodium butyrate, using Anti-Histone H4 (acetyl Lys5) Antibody [RM199] (red). Actin filaments have been labeled with fluorescein phalloidin (green).