Western Blot - Anti-Histone H4 (acetyl Lys5) Antibody (A306101)
Western blot analysis of extracts of various cell lines, using Anti-Histone H4 (acetyl Lys5) Antibody (A306101) at 1:1,000 dilution. HeLa cells, NIH/3T3 cells and C6 cells were treated by TSA (1 uM) at 37°C for 18 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Chromatin immunoprecipitation (ChIP) analysis of extracts of HeLa cells, using Anti-Histone H4 (acetyl Lys5) Antibody (A306101) and Rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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