Recombinant Anti-Histone H4 (acetyl Lys20) Antibody [RM205] (A121200)

Western blot of (1) acid extracts of HeLa cells, and (2) recombinant Histone H4, using Anti-Histone H4 (acetyl Lys20) Antibody [RM205] at 1µg/ml, showed a band of Histone H4 acetylated at Lysine 20 in HeLa cells.

Anti-Histone H4 (acetyl Lys20) Antibody [RM205] specifically reacts to Histone H4 acetylated at Lysine 20 (K20ac). There is no cross reactivity with unmodified Lysine 20 (K20 ctrl), acetylated Lysine 5 (K5ac), Lysine 8 (K8ac), Lysine 12 (K12ac), Lysine 16(K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.

Immunocytochemical staining of HeLa cells treated with sodium butyrate, using Anti-Histone H4 (acetyl Lys20) Antibody [RM205] (red). Actin filaments have been labeled with fluorescein phalloidin (green).