Anti-Histone H4 (acetyl Lys16) Antibody (A88199)

Western blot analysis of various lysates, using Anti-Histone H4 (acetyl Lys16) Antibody (A88199) at 1:400 dilution. NIH/3T3 and C6 cells were treated by TSA (1 uM) at 37°C for 18 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

Immunofluorescence analysis of C6 cells treated by TSA (upper left) and untreated C6 cells (upper right) using Anti-Histone H4 (acetyl Lys16) Antibody (A88199), at a dilution of 1:100, (Red). DAPI was used to stain the cell nuclei (blue).

Immunofluorescence analysis of C6 cells using Anti-Histone H4 (acetyl Lys16) Antibody (A88199) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue). C6 cells were treated by TSA (1 uM) at 37°C for 18 hours. DAPI was used to stain the cell nuclei (blue).

Immunofluorescence analysis of NIH/3T3 cells using Anti-Histone H4 (acetyl Lys16) Antibody (A88199) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue). NIH/3T3 cells were treated by TSA (1 uM) at 37°C for 18 hours. DAPI was used to stain the cell nuclei (blue).