Recombinant Anti-Histone H4 (acetyl Lys12) Antibody [RM202] (A121202)

Anti-Histone H4 (acetyl Lys12) Antibody [RM202] specifically reacts to Histone H4 acetylated at Lysine 12 (K12ac). There is no cross reactivity with unmodified Lysine 16 (K16 ctrl), acetylated Lysine 5 (K5ac), Lysine 8 (K8ac), Lysine 16 (K16ac), Lysine 20 (K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.

Western blot of acid extracts from (1) HeLa cells treated with sodium butyrate and (2) HeLa cells untreated, using Anti-Histone H4 (acetyl Lys12) Antibody [RM202] at 0.5µg/ml, showed a band of Histone H4 acetylated Lysine 12 in HeLa cells.

Immunocytochemical staining of HeLa cells treated with sodium butyrate, using Anti-Histone H4 (acetyl Lys12) Antibody [RM202] (red). Actin filaments have been labeled with fluorescein phalloidin (green).