Anti-Histone H4 (acetyl Lys12) Antibody (A91511)

Name: Anti-Histone H4 (acetyl Lys12) Antibody
Brand: Antibodies.com
SKU: A91511
Price: 485.0 USD
Availability: InStock

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$485

Rabbit polyclonal antibody to Histone H4 (acetyl Lys12) for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Tags:

Nuclear Marker

Specifications

Name

Anti-Histone H4 (acetyl Lys12) Antibody

Description

Rabbit polyclonal antibody to Histone H4 (acetyl Lys12).

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic acetylated peptide around K12 of human Histone H4 (NP_001029249.1).

Sequence

GLGKG

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

11 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

H4/A, H4C1, H4FA, HIST1H4A

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (7)

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Western Blot - Anti-Histone H4 (acetyl Lys12) Antibody (A91511)

Western blot analysis of extracts of various cell lines, using Anti-Histone H4 (acetyl Lys12) Antibody (A91511) at 1:500 dilution. HeLa cells and C6 cells were treated by TSA (1 uM) at 37°C for 18 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Immunohistochemistry - Anti-Histone H4 (acetyl Lys12) Antibody (A91511)

Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-Histone H4 (acetyl Lys12) Antibody (A91511) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Histone H4 (acetyl Lys12) Antibody (A91511)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-Histone H4 (acetyl Lys12) Antibody (A91511) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Histone H4 (acetyl Lys12) Antibody (A91511)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-Histone H4 (acetyl Lys12) Antibody (A91511) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.