Anti-Histone H3 (tri methyl Lys36) Antibody [ARC50050] (A306148)

Name: Anti-Histone H3 (tri methyl Lys36) Antibody [ARC50050]
Brand: Antibodies.com
SKU: A306148
Price: 540.0 USD
Availability: InStock

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$540

Rabbit monoclonal antibody to Histone H3 (tri methyl Lys36) for WB, IHC, IP, ChIP, ChIP-seq and Dot Blot.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

Nuclear Loading Control

Specifications

Name

Anti-Histone H3 (tri methyl Lys36) Antibody [ARC50050]

Description

Rabbit monoclonal antibody to Histone H3 (tri methyl Lys36).

Applications

WB, IHC, IP, ChIP, ChIP-seq, Dot Blot

Dilutions

DB: 1:500-1:1,000, WB: 1:500-1:1,000, IP: 1:500-1:1,000, IHC: 1:50-1:200, ChIP: 1:50-1:200, ChIP-seq: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic trimethylated peptide around K36 of human Histone H3 (NP_003520.1).

Sequence

GVKKP

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC50050

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

17 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

H3-3A, H3-5, H3-clustered histone 13, H3-clustered histone 14, H3-clustered histone 15, H3.3A, H3C1, H3C15, H3F3, H3F3A, H3F3C, H3FA, HIST1H3A, HIST2H3A, Histone H3.1, Histone H3.2, Histone H3.3, Histone H3.3C, Histone H3.5, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/m, Histone H3/o

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Chromatin Immunoprecipitation - Anti-Histone H3 (tri methyl Lys36) Antibody [ARC50050] (A306148)

Chromatin immunoprecipitations analysis of cross-linked chromatin from HeLa cells, using Anti-Histone H3 (tri methyl Lys36) Antibody [ARC50050] (A306148). The ChIP sequencing results indicate the enrichment pattern of H3K36me3 in selected genomic region and representative gene loci (GAPDH), as shown in figure.

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Western Blot - Anti-Histone H3 (tri methyl Lys36) Antibody [ARC50050] (A306148)

Western blot analysis of extracts of various cell lines, using Anti-Histone H3 (tri methyl Lys36) Antibody [ARC50050] (A306148) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Dot Blot - Anti-Histone H3 (tri methyl Lys36) Antibody [ARC50050] (A306148)

Dot blot analysis of a mixture of peptides using Anti-Histone H3 (tri methyl Lys36) Antibody [ARC50050] (A306148) at a 1:1,000 dilution.

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Western Blot - Anti-Histone H3 (tri methyl Lys36) Antibody [ARC50050] (A306148)

Immunoprecipitation analysis of 600µg extracts of 293F cells using 5µg of Anti-Histone H3 (tri methyl Lys36) Antibody [ARC50050] (A306148). This Western blot was performed on the immunoprecipitate using Anti-Histone H3 (tri methyl Lys36) Antibody [ARC50050] (A306148) at a dilution of 1:1000.

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Anti-Histone H3 (tri methyl Lys36) Antibody [ARC50050] (A306148)

Rabbit monoclonal antibody to Histone H3 (tri methyl Lys36) for WB, IHC, IP, ChIP, ChIP-seq and Dot Blot.

Shipping Information

Specifications

Scientific Validation DataValidation Data (4)

Chromatin Immunoprecipitation - Anti-Histone H3 (tri methyl Lys36) Antibody [ARC50050] (A306148)

Western Blot - Anti-Histone H3 (tri methyl Lys36) Antibody [ARC50050] (A306148)

Dot Blot - Anti-Histone H3 (tri methyl Lys36) Antibody [ARC50050] (A306148)

Western Blot - Anti-Histone H3 (tri methyl Lys36) Antibody [ARC50050] (A306148)

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