Anti-Histone H3 (mono methyl Lys79) Antibody (A16715)

Name: Anti-Histone H3 (mono methyl Lys79) Antibody
Brand: Antibodies.com
SKU: A16715
Price: 435.0 USD
Availability: InStock

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$435

Rabbit polyclonal antibody to Histone H3 (mono methyl Lys79) for WB, IHC, ICC/IF, IP, ChIP and ChIP-seq.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

Nuclear Loading Control

Specifications

Name

Anti-Histone H3 (mono methyl Lys79) Antibody

Description

Rabbit polyclonal antibody to Histone H3 (mono methyl Lys79).

Applications

WB, IHC, ICC/IF, IP, ChIP, ChIP-seq

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:50-1:200, ChIP: 1:20-1:100, ChIP-seq: 1:20-1:100

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic monomethylated peptide around K79 of human histone H3 (NP_003520.1).

Sequence

DFKTD

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

17 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

H3-3A, H3-5, H3-clustered histone 13, H3-clustered histone 14, H3-clustered histone 15, H3.3A, H3C1, H3C15, H3F3, H3F3A, H3F3C, H3FA, HIST1H3A, HIST2H3A, Histone H3.1, Histone H3.2, Histone H3.3, Histone H3.3C, Histone H3.5, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/m, Histone H3/o

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-Histone H3 (mono methyl Lys79) Antibody (A16715)

Western blot analysis of extracts of various cell lines, using Anti-Histone H3 (mono methyl Lys79) Antibody (A16715) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 300s.

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Dot Blot - Anti-Histone H3 (mono methyl Lys79) Antibody (A16715)

Dot blot analysis of a mixture of methylation peptides using Anti-Histone H3 (mono methyl Lys79) Antibody (A16715).

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Immunofluorescence - Anti-Histone H3 (mono methyl Lys79) Antibody (A16715)

Immunofluorescence analysis of 293T cells using Anti-Histone H3 (mono methyl Lys79) Antibody (A16715). DAPI was used to stain the cell nuclei (blue).

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Anti-Histone H3 (mono methyl Lys79) Antibody (A16715)

Rabbit polyclonal antibody to Histone H3 (mono methyl Lys79) for WB, IHC, ICC/IF, IP, ChIP and ChIP-seq.

Shipping Information

Specifications

Scientific Validation DataValidation Data (3)

Western Blot - Anti-Histone H3 (mono methyl Lys79) Antibody (A16715)

Dot Blot - Anti-Histone H3 (mono methyl Lys79) Antibody (A16715)

Immunofluorescence - Anti-Histone H3 (mono methyl Lys79) Antibody (A16715)

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