Recombinant Anti-Histone H3 Antibody [RM186] (A121358)

Name: Anti-Histone H3 Antibody [RM186]
Brand: Antibodies.com
SKU: A121358
Price: 585.0 USD
Availability: InStock

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$585

Recombinant rabbit monoclonal [RM186] antibody to Histone H3 for WB, ELISA, Multiplex Protein Detection and ICC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 4-6 business days.

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Recombinant Antibody Nuclear Loading Control

Specifications

Name

Anti-Histone H3 Antibody [RM186]

Description

Recombinant rabbit monoclonal [RM186] antibody to Histone H3.

Specificity

This antibody recognizes Histone H3 that is unmodified at Lys4 only. It does not recognise acetylated or methylated Lys4.

Applications

WB, ELISA, Multiplex Protein Detection, ICC

Dilutions

WB: 1-2 µg/ml, ELISA: 0.5-1 µg/ml, Multiplex Protein Detection: 0.5-2 µg/ml

Reactivity

All Vertebrates

Immunogen

Synthetic peptide corresponding to Histone H3 (N terminal).

Host

Rabbit

Clonality

Monoclonal

Clone ID

RM186

Isotype

IgG

Conjugate

Unconjugated

Purification

Protein A chromatography.

Concentration

1 mg/ml

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

H3-3A, H3-5, H3-clustered histone 13, H3-clustered histone 14, H3-clustered histone 15, H3.3A, H3C1, H3C15, H3F3, H3F3A, H3F3C, H3FA, HIST1H3A, HIST2H3A, Histone H3.1, Histone H3.2, Histone H3.3, Histone H3.3C, Histone H3.5, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/m, Histone H3/o

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Anti-Histone H3 Antibody [RM186] (A121358)

Anti-Histone H3 Antibody [RM186] specifically recognizes Histone H3 unmodified at Lys4 and does not recognize acetylated, mono-methylated, di methylated, or tri-methylated Lys4. The antibody binding specificity allows for modifications of Arg2 or Thr3 in Histone H3.

Enlarge Image

Western Blot - Anti-Histone H3 Antibody [RM186] (A121358)

Western blot of acid extracts of HeLa cells treated with sodium butyrate (HeLa + NaBu), treated with Nocodazole (HeLa + Noc), or non-treated, and recombinant Histone H3.1 protein, using Anti-Histone H3 Antibody [RM186] at 1µg/ml, showed a band of Histone H3 with unmodified Lys4.

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Immunocytochemistry - Anti-Histone H3 Antibody [RM186] (A121358)

Immunocytochemical staining of HeLa cells treated with sodium butyrate, using Anti-Histone H3 Antibody [RM186] (red). Actin filaments have been labeled with fluorescein phalloidin (green).

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See all Histone H3 Antibodies

Recombinant Anti-Histone H3 Antibody [RM186] (A121358)

Recombinant rabbit monoclonal [RM186] antibody to Histone H3 for WB, ELISA, Multiplex Protein Detection and ICC.

Shipping Information

Specifications

Scientific Validation DataValidation Data (3)

Anti-Histone H3 Antibody [RM186] (A121358)

Western Blot - Anti-Histone H3 Antibody [RM186] (A121358)

Immunocytochemistry - Anti-Histone H3 Antibody [RM186] (A121358)

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