Anti-Histone H3 Antibody [AMC0015] (A91520)

Name: Anti-Histone H3 Antibody [AMC0015]
Brand: Antibodies.com
SKU: A91520
Price: 485.0 USD
Availability: InStock

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$485

Mouse monoclonal [AMC0015] antibody to Histone H3 for WB, IHC-P, ICC/IF, IP, ChIP, ELISA, Dot Blot and CUT&Tag.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Tags:

Nuclear Loading Control

Specifications

Name

Anti-Histone H3 Antibody [AMC0015]

Description

Mouse monoclonal [AMC0015] antibody to Histone H3.

Applications

WB, IHC-P, ICC/IF, IP, ChIP, ELISA, Dot Blot, CUT&Tag

Positive Control

HeLa, NIH/3T3, C6, H3 protein.

Dilutions

WB: 1:500-1:1,000, Dot Blot: 1:500-1:2,000, IHC-P: 1:50-1:200, ICC/IF: 1:50-1:200, ELISA: 1 µg/ml (starting concentration), ChIP: 5µg antibody for 5µg-10µg of Chromatin, CUT&Tag: 105 cells/1 µg.

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic methylated peptide corresponding to residues surrounding K27 of human Histone H3.

Host

Mouse

Clonality

Monoclonal

Clone ID

AMC0015

Isotype

IgG1

Light Chains

kappa

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

17 kDa

Predicted MW

16 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% ProClin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

H3-3A, H3-5, H3-clustered histone 13, H3-clustered histone 14, H3-clustered histone 15, H3.3A, H3C1, H3C15, H3F3, H3F3A, H3F3C, H3FA, HIST1H3A, HIST2H3A, Histone H3.1, Histone H3.2, Histone H3.3, Histone H3.3C, Histone H3.5, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/m, Histone H3/o

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (10)

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Anti-Histone H3 Antibody [AMC0015] (A91520)

CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina from 10^5 K562 cells with 1 µg Anti-Histone H3 Antibody [AMC0015] (A91520) and Goat Anti-Mouse IgG (H+L). The CUT&Tag results indicate the enrichment pattern of Histone H3 in representative gene loci (MYOD1), as shown in figure.

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Western Blot - Anti-Histone H3 Antibody [AMC0015] (A91520)

Histone H3 expression in various lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-Histone H3 Antibody [AMC0015] (A91520) at a dilution of 1:1000 and detected with chemiluminescence.

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Dot Blot - Anti-Histone H3 Antibody [AMC0015] (A91520)

Dot blot analysis of a mixture of methylation peptides using Anti-Histone H3 Antibody [AMC0015] (A91520) at a dilution of 1:1000.

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Immunohistochemistry - Anti-Histone H3 Antibody [AMC0015] (A91520)

Histone H3 expression in human colon carcinoma tissue analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by microwaving in 10 mM PBS buffer, pH 7.2. Staining was performed with Anti-Histone H3 Antibody [AMC0015] (A91520) at a dilution of 1:25.