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Recombinant Anti-Histone H3 (acetyl Lys9) Antibody [RM161] (A121229)

Multiplex Immunoassay - Anti-Histone H3 (acetyl Lys9) Antibody [RM161] (A121229) - Antibodies.com Western Blot - Anti-Histone H3 (acetyl Lys9) Antibody [RM161] (A121229) - Antibodies.com ELISA - Anti-Histone H3 (acetyl Lys9) Antibody [RM161] (A121229) - Antibodies.com Chromatin Immunoprecipitation - Anti-Histone H3 (acetyl Lys9) Antibody [RM161] (A121205) - Antibodies.com Immunocytochemistry - Anti-Histone H3 (acetyl Lys9) Antibody [RM161] (A121229) - Antibodies.com Immunohistochemistry - Anti-Histone H3 (acetyl Lys9) Antibody [RM161] (A121229) - Antibodies.com
Recombinant Antibody
$565

Recombinant rabbit monoclonal [RM161] antibody to Histone H3 (acetyl Lys9) for WB, ELISA, Multiplex Protein Detection, ChIP, ICC and IHC.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.

  • Specifications

Name
Anti-Histone H3 (acetyl Lys9) Antibody [RM161]
Description
Recombinant rabbit monoclonal [RM161] antibody to Histone H3 (acetyl Lys9).
Specificity
This antibody recognizes Histone H3 acetylated at Lysine 9.
Applications
WB, ELISA, Multiplex Protein Detection, ChIP, ICC, IHC
Dilutions
WB: 0.25-1 µg/ml, ICC: 0.5-2 µg/ml, ChIP: 2-10 µg/ml, IHC: 0.1-1 µg/ml, ELISA: 0.2-1 µg/ml, Multiplex Protein Detection: 0.05-0.5 µg/ml
Reactivity
All Vertebrates
Cross Reactivity
There is no cross reactivity with other acetylated Lysines in Histone H3.
Immunogen
Synthetic peptide corresponding to Histone H3 (acetyl Lys9).
Host
Rabbit
Clonality
Monoclonal
Clone ID
RM161
Isotype
IgG
Conjugate

Unconjugated

Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
H3-3A, H3-5, H3-clustered histone 13, H3-clustered histone 14, H3-clustered histone 15, H3.3A, H3C1, H3C15, H3F3, H3F3A, H3F3C, H3FA, HIST1H3A, HIST2H3A, Histone H3.1, Histone H3.2, Histone H3.3, Histone H3.3C, Histone H3.5, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/m, Histone H3/o
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (6)

Multiplex Immunoassay - Anti-Histone H3 (acetyl Lys9) Antibody [RM161] (A121229) - Antibodies.comEnlarge Image

Anti-Histone H3 (acetyl Lys9) Antibody [RM161] (A121229)

Anti-Histone H3 (acetyl Lys9) Antibody [RM161] specifically reacts to Histone H3 acetylated at Lysine 9 (K9ac). There is no cross reactivity with acetylated Lysine 4 (K4ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), Lysine 79 (K79ac), or Lysine 122 (K122) in Histone H3.
Western Blot - Anti-Histone H3 (acetyl Lys9) Antibody [RM161] (A121229) - Antibodies.comEnlarge Image

Western Blot - Anti-Histone H3 (acetyl Lys9) Antibody [RM161] (A121229)

Western blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+), using Anti-Histone H3 (acetyl Lys9) Antibody [RM161] at 0.25µg/ml, showed a band of Histone H3 acetylated Lysine 9 in treated HeLa cells.
ELISA - Anti-Histone H3 (acetyl Lys9) Antibody [RM161] (A121229) - Antibodies.comEnlarge Image

Anti-Histone H3 (acetyl Lys9) Antibody [RM161] (A121229)

Sandwich ELISA against acetylated Histone H3 at Lys9 using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. 1µg/ml of Anti-pan Histone H3 Antibody [RM188] was used as the capture antibody and 1µg/ml of Anti-Histone H3 (acetyl Lys9) Antibody [RM161] (Biotin) was used as the detection antibody.
Chromatin Immunoprecipitation - Anti-Histone H3 (acetyl Lys9) Antibody [RM161] (A121205) - Antibodies.comEnlarge Image

Chromatin Immunoprecipitation - Anti-Histone H3 (acetyl Lys9) Antibody [RM161] (A121229)

ChIP performed on HeLa cells using 5µg of Anti-Histone H3 (acetyl Lys9) Antibody [RM161]. Real-time PCR was performed using primers specific to the gene indicated.
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