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Anti-Histone H2A.X (phospho Ser139) Antibody (A93597)

ELISA - Anti-Histone H2A.X (phospho Ser139) Antibody (P12-1116) - Antibodies.com Western Blot - Anti-Histone H2A.X (phospho Ser139) Antibody (P12-1116) - Antibodies.com
This product is discontinued

Anti-Histone H2A.X (phospho Ser139) Antibody (A93597) has been discontinued and is no longer available.

View all available products.

  • Specifications

Name
Anti-Histone H2A.X (phospho Ser139) Antibody
Description
Rabbit polyclonal antibody to Histone H2A.X (phospho Ser139).
Specificity
This antibody detects endogenous levels of H2AFX only when phosphorylated at Ser139.
Applications
WB, ELISA
Dilutions
WB: 1:500-1:1000, IF: 1:100-1:500, ELISA: 1:10000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phospho-peptide derived from human Histone H2A.X around the phosphorylation site of Ser139.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
15kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

ELISA - Anti-Histone H2A.X (phospho Ser139) Antibody (P12-1116) - Antibodies.comEnlarge Image

Anti-Histone H2A.X (phospho Ser139) Antibody (A93597)

ELISA for immunogen phosphopeptide (left) and non-phosphopeptide (right) using Anti-Histone H2A.X (phospho Ser139) Antibody.
Western Blot - Anti-Histone H2A.X (phospho Ser139) Antibody (P12-1116) - Antibodies.comEnlarge Image

Western Blot - Anti-Histone H2A.X (phospho Ser139) Antibody (A93597)

Western blot analysis of lysates from 293 cells treated with heat shock using Anti-Histone H2A.X (phospho Ser139) Antibody.