Rabbit polyclonal antibody to Histone H2A.X (Phospho-Ser139)
Specificity
The antibody detects endogenous level of Histone H2A.X only when phosphorylated at serine 139.
Applications
WB, IF
Dilutions
Western blotting: 1:500~1:1000 Immunofluorescence: 1:100~1:200
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of serine 139 (Q-A-S(p)-Q-E) derived from Human Histone H2A.X.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Predicted MW
15kd
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Western blot analysis of extracts from 293 cells, treated with UV+serum or calf intestinal phosphatase (CIP), using Histone H2A.X (Phospho-Ser139) Antibody #11268.
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