Anti-Histone H2A.X (phospho Ser139) Antibody (A16444)

Name: Anti-Histone H2A.X (phospho Ser139) Antibody
Brand: Antibodies.com
SKU: A16444
Price: 485.0 USD
Availability: InStock

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$485

Rabbit polyclonal antibody to Histone H2A.X (phospho Ser139) for WB.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Histone H2A.X (phospho Ser139) Antibody

Description

Rabbit polyclonal antibody to Histone H2A.X (phospho Ser139).

Applications

Dilutions

WB: 1:100-1:500

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S139 of human Histone H2AX (NP_002096.1).

Sequence

TQASQ

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

17 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

H2a/x, H2AFX, H2AX, Histone H2AX

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-Histone H2A.X (phospho Ser139) Antibody (A16444)

Western blot analysis of 293T, using Anti-Histone H2A.X (phospho Ser139) Antibody (A16444) at 1:500 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Western Blot - Anti-Histone H2A.X (phospho Ser139) Antibody (A16444)

Western blot analysis of NIH/3T3, using Anti-Histone H2A.X (phospho Ser139) Antibody (A16444) at 1:500 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Western Blot - Anti-Histone H2A.X (phospho Ser139) Antibody (A16444)

Western blot analysis of C6, using Anti-Histone H2A.X (phospho Ser139) Antibody (A16444) at 1:500 dilution. C6 cells were treated by UV at room temperature for 15-30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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