Anti-Histone H2A.X Antibody (A13852)

Name: Anti-Histone H2A.X Antibody
Brand: Antibodies.com
SKU: A13852
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to Histone H2A.X for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Histone H2A.X Antibody

Description

Rabbit polyclonal antibody to Histone H2A.X.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S2 of human Histone H2AX (NP_002096.1).

Sequence

MSGRG

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

15 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

H2a/x, H2AFX, H2AX, Histone H2AX

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-Histone H2A.X Antibody (A13852)

Western blot analysis of extracts of various cell lines, using Anti-Histone H2A.X Antibody (A13852) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Immunofluorescence - Anti-Histone H2A.X Antibody (A13852)

Confocal immunofluorescence analysis of HeLa cells using Anti-Histone H2A.X Antibody (A13852) at a dilution of 1:200. DAPI was used to stain the cell nuclei (blue).

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