Rabbit polyclonal antibody to Histone H2A.X (Ab-139)
Specificity
The antibody detects endogenous level of total Histone H2A.X protein.
Applications
WB, IHC, IF
Reactivity
Human
Immunogen
Peptide sequence around aa.137~141 (Q-A-S-Q-E) derived from Human Histone H2A.X.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Histone H2A.X (Ab-139) Antibody #21260 (left) or the same antibody preincubated with blocking peptide (right).
![Western Blot - Anti-Histone H2A.X (phospho Ser139) Antibody [ARC0110] (A308961) - Antibodies.com](https://cdn.antibodies.com/image/catalog/308/A308961_1.jpg?profile=product_alternative)
![Western Blot - Anti-Histone H2AX Antibody [RM214] (A121318) - Antibodies.com](https://cdn.antibodies.com/image/catalog/121/A121318_1.png?profile=product_alternative)
