Anti-Histone H2A Antibody [ARC0253] (A306399)

Name: Anti-Histone H2A Antibody [ARC0253]
Brand: Antibodies.com
SKU: A306399
Price: 540.0 USD
Availability: InStock

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$540

Rabbit monoclonal [ARC0253] antibody to Histone H2A for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

Nuclear Marker

Specifications

Name

Anti-Histone H2A Antibody [ARC0253]

Description

Rabbit monoclonal [ARC0253] antibody to Histone H2A.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic hydroxylated peptide around Y39 of human Histone H2A (P04908).

Sequence

KGNYS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0253

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

17 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

H2A-clustered histone 1, H2A-clustered histone 12, H2A-clustered histone 18, H2A-clustered histone 19, H2A-clustered histone 20, H2A-clustered histone 21, H2A-clustered histone 25, H2A-clustered histone 6, H2A.1, H2AC1, H2AC11, H2AC12, H2AC14, H2AC18, H2AC20, H2AC21, H2AC25, H2AC4, H2AC6, H2AC7, H2AFE, H2AFG, H2AFL, H2AFM, H2AFO, H2AFP, H2AFQ, H2AFR, H2AW, HIST1H2AA, HIST1H2AB, HIST1H2AC, HIST1H2AD, HIST1H2AG, HIST1H2AH, HIST1H2AI, HIST1H2AJ, HIST2H2AA, HIST2H2AA3, HIST2H2AB, HIST2H2AC, HIST3H2A, Histone H2A type 1, Histone H2A type 1-A, Histone H2A type 1-B/E, Histone H2A type 1-C, Histone H2A type 1-D, Histone H2A type 1-H, Histone H2A type 1-J, Histone H2A type 2-A, Histone H2A type 2-B, Histone H2A type 2-C, Histone H2A type 3, Histone H2A-GL101, Histone H2A.2, Histone H2A.3, Histone H2A/a, Histone H2A/e, Histone H2A/g, Histone H2A/l, Histone H2A/m, Histone H2A/o, Histone H2A/ptl, Histone H2A/q, Histone H2A/r, Histone H2A/s

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-Histone H2A Antibody [ARC0253] (A306399)

Western blot analysis of extracts of various cell lines, using Anti-Histone H2A Antibody [ARC0253] (A306399) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Western Blot - Anti-Histone H2A Antibody [ARC0253] (A306399)

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Immunohistochemistry - Anti-Histone H2A Antibody [ARC0253] (A306399)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Histone H2A Antibody [ARC0253] (A306399) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Histone H2A Antibody [ARC0253] (A306399)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Histone H2A Antibody [ARC0253] (A306399) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.