Anti-Histone H2A (acetyl Lys5) Antibody (A91516)

Name: Anti-Histone H2A (acetyl Lys5) Antibody
Brand: Antibodies.com
SKU: A91516
Price: 435.0 USD
Availability: InStock

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$435

Rabbit polyclonal antibody to Histone H2A (acetyl Lys5) for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

Nuclear Marker

Specifications

Name

Anti-Histone H2A (acetyl Lys5) Antibody

Description

Rabbit polyclonal antibody to Histone H2A (acetyl Lys5).

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic acetylated peptide around K5 of human Histone H2A (NP_003508.1).

Sequence

GRGKQ

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

14 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

H2A-clustered histone 1, H2A-clustered histone 12, H2A-clustered histone 18, H2A-clustered histone 19, H2A-clustered histone 20, H2A-clustered histone 21, H2A-clustered histone 25, H2A-clustered histone 6, H2A.1, H2AC1, H2AC11, H2AC12, H2AC14, H2AC18, H2AC20, H2AC21, H2AC25, H2AC4, H2AC6, H2AC7, H2AFE, H2AFG, H2AFL, H2AFM, H2AFO, H2AFP, H2AFQ, H2AFR, H2AW, HIST1H2AA, HIST1H2AB, HIST1H2AC, HIST1H2AD, HIST1H2AG, HIST1H2AH, HIST1H2AI, HIST1H2AJ, HIST2H2AA, HIST2H2AA3, HIST2H2AB, HIST2H2AC, HIST3H2A, Histone H2A type 1, Histone H2A type 1-A, Histone H2A type 1-B/E, Histone H2A type 1-C, Histone H2A type 1-D, Histone H2A type 1-H, Histone H2A type 1-J, Histone H2A type 2-A, Histone H2A type 2-B, Histone H2A type 2-C, Histone H2A type 3, Histone H2A-GL101, Histone H2A.2, Histone H2A.3, Histone H2A/a, Histone H2A/e, Histone H2A/g, Histone H2A/l, Histone H2A/m, Histone H2A/o, Histone H2A/ptl, Histone H2A/q, Histone H2A/r, Histone H2A/s

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-Histone H2A (acetyl Lys5) Antibody (A91516)

Western blot analysis of extracts of various cell lines, using Anti-Histone H2A (acetyl Lys5) Antibody (A91516) at 1:1,000 dilution. C2C12 cells and C6 cells were treated by TSA (1 uM) at 37°C for 18 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Immunofluorescence - Anti-Histone H2A (acetyl Lys5) Antibody (A91516)

Immunofluorescence analysis of C6 cells using Anti-Histone H2A (acetyl Lys5) Antibody (A91516) at a dilution of 1:100. C6 cells were treated by TSA (1 uM) at 37°C for 18 hours. DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-Histone H2A (acetyl Lys5) Antibody (A91516)

Immunofluorescence analysis of HeLa cells using Anti-Histone H2A (acetyl Lys5) Antibody (A91516) at a dilution of 1:100. HeLa cells were treated by TSA (1 uM) at 37°C for 18 hours. DAPI was used to stain the cell nuclei (blue).

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