Anti-Histone H1t Antibody (A308060)

Name: Anti-Histone H1t Antibody
Brand: Antibodies.com
SKU: A308060
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to Histone H1t for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Histone H1t Antibody

Description

Rabbit polyclonal antibody to Histone H1t.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 150-207 of human Histone H1t (NP_005314.2).

Sequence

NKRAKKPRATTPKTVRSGRKAKGAKGKQQQKSPVKARASKSKLTQHHEVNVRKATSKK

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

30 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

H1-0, H1-1, H1-10, H1-3, H1-5, H1-6, H1-8, H1F0, H1F1, H1F3, H1F5, H1FOO, H1FT, H1FV, H1FX, H1OO, H1T, HIST1H1A, HIST1H1B, HIST1H1D, HIST1H1T, Histone H1', Histone H1(0), Histone H1.0, Histone H1.1, Histone H1.10, Histone H1.3, Histone H1.5, Histone H1.8, Histone H1a, Histone H1b, Histone H1c, Histone H1oo, Histone H1s-2, Histone H1s-3, Histone H1x, Oocyte-specific histone H1, Oocyte-specific linker histone H1, osH1, Testicular H1 histone

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-Histone H1t Antibody (A308060)

Western blot analysis of extracts of various cell lines, using Anti-Histone H1t Antibody (A308060) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Immunohistochemistry - Anti-Histone H1t Antibody (A308060)

Immunohistochemistry analysis of paraffin-embedded human esophageal using Anti-Histone H1t Antibody (A308060) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Histone H1t Antibody (A308060)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Histone H1t Antibody (A308060) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Histone H1t Antibody (A308060)

Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-Histone H1t Antibody (A308060) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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