Anti-Histone H1 Antibody [AE-4] (A279650)

Anti-Histone H1 Antibody [AE-4]

Mouse monoclonal [AE-4] antibody to Histone H1.

This antibody recognises Histone H1.The histone proteins serve as a structural scaffold for the organization of nuclear DNA into chromatin. In eukaryotic cells there are 4 core histone proteins, H2A, H2B, H3 and H4 that assemble to form an octamer round which the DNA is wrapped in a sequence dependant manner. The resulting complex is known as a nucleosome and is the basic subunit of chromatin. Histone H1 acts as a linker and binds to each nucleosome, and by self affinity, links these nucleosomes together resulting in the 30 nm chromatin fiber.In blots of rat brain homogenate a duplet is observed at ~27-29 kDa consistent with Histone H1. Speckled staining is observed in the nucleus of normal and malignant cells.

WB, IHC-P, IHC-Fr, IF, IP

WB: 1:100

Human

Nuclei from myeloid leukamia biopsy cells.

Mouse

Monoclonal

AE-4

IgG2a

Protein G affinity chromatography of tissue culture supernatant.

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human Histone H1 antibody, clone AE-4 recognizes histone H1.The histone proteins serve as a structural scaffold for the organization of nuclear DNA into chromatin. In eukaryotic cells there are 4 core histone proteins, H2A, H2B, H3 and H4 that assemble to form an octamer round which the DNA is wrapped in a sequence dependant manner. The resulting complex is known as a nucleosome and is the basic subunit of chromatin. Histone H1 acts as a linker and binds to each nucleosome, and by self affinity, links these nucleosomes together resulting in the 30 nm chromatin fiber.In blots of rat brain homogenate a duplet is observed at ~27-29 kDa consistent with Histone H1. Speckled staining is observed in the nucleus of normal and malignant cells.

H1-0, H1-1, H1-10, H1-3, H1-5, H1-6, H1-8, H1F0, H1F1, H1F3, H1F5, H1FOO, H1FT, H1FV, H1FX, H1OO, H1T, HIST1H1A, HIST1H1B, HIST1H1D, HIST1H1T, Histone H1', Histone H1(0), Histone H1.0, Histone H1.1, Histone H1.10, Histone H1.3, Histone H1.5, Histone H1.8, Histone H1a, Histone H1b, Histone H1c, Histone H1oo, Histone H1s-2, Histone H1s-3, Histone H1t, Histone H1x, Oocyte-specific histone H1, Oocyte-specific linker histone H1, osH1, Testicular H1 histone