Recombinant rabbit monoclonal [DM1] antibody to His Tag.
Applications
ELISA, WB
Dilutions
WB: 1:1,000
Host
Rabbit
Clonality
Monoclonal
Clone ID
DM1
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Western Blot - Anti-His Tag Antibody [DM1] - BSA and Azide free (A317439)
Western blot analysis of His-Tag fusion protein using Anti-His Tag Antibody [DM1] - Azide free (A317439) at 1:1,000 dilution. Secondary Antibody: Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:5,000 dilution. His-tag fusion protein: 70kDa, 20ng per lane. Blocking buffer: 5% non-fat dry milk in TBST. Exposure time: 1s.
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