Mouse monoclonal [ABD2.2.20] antibody to His Tag (HRP).
Specificity
This antibody recognises proteins and peptides containing the H-H-H-H-H or H-H-H-H-H-H motif and is therefore of value in detecting proteins containing 5x (penta) his and 6x (hexa) his tags. This antibody will detect any accessible N-terminal, C-terminal or internal histidine tags and is highly recommended for use in assay development for the detection of his-tagged HuCAL primary antibodies.
Applications
WB, ELISA
Dilutions
ELISA: 1:1,000 - 1:2,000, WB: 1:1,000 - 1:2,000
Reactivity
Species Independent
Host
Mouse
Clonality
Monoclonal
Clone ID
ABD2.2.20
Isotype
IgG1
Conjugate
HRP
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline.
Storage
Store undiluted at 4°C. Do not freeze! Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti penta histidine tag antibody, clone ABD2.2.20, recognizes proteins and peptides containing the H-H-H-H-H or H-H-H-H-H-H motif and is therefore of value in detecting proteins containing 5x (penta) his and 6x (hexa) his tags. This antibody will detect any accessible N-terminal, C-terminal or internal histidine tags and is highly recommended for use in assay development for the detection of his-tagged HuCAL primary antibodies.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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