Anti-HIF-1 alpha Antibody [Halpha111a] (A280568)

Anti-HIF-1 alpha Antibody [Halpha111a]

Mouse monoclonal [Halpha111a] antibody to HIF-1 alpha.

This antibody recognises human Hypoxia-inducible factor 1-alpha otherwise known as HIF1 alpha (HIF1A) or ARNT interacting protein, a widely expressed bHLH-PAS transcription factor, which acts as a critical regulatory protein in the host response to hypoxia. HIF1A and HIF1B, also known as ARNT form the highly-conserved heterodimeric HIF-1 transcriptional complex. During hypoxic conditions, HIF1A accumulates in the nucleus and activates the transcription of many genes encoding proteins involved in the production of oxygen delivery and metabolic adaptation, such as those for vascular endothelial growth factor (VEGFA). Under normal oxygen conditions, HIF1A is targeted by hypoxia inducible factor prolyl-hydroxylases, followed by rapid protease degradation, which is inhibited during hypoxia (Hirsilä et al. 2003). Overexpression of HIF1A occurs in many common human cancers, including pancreatic, bladder and renal carcinomas. Mouse anti Human HIF1a, clone Halpha111a has been successfully used for the identifcation of HIF1a in human samples by both immunohistochemistry and western blotting.

WB, IHC-P

IHC-P 1: 1:25

Human

Recombinant human HIF1 alpha.

Mouse

Monoclonal

Halpha111a

IgG2a

Protein A affinity chromatography of tissue culture supernatant.

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human HIF1 alpha antibody, clone Halpha111a recognizes human Hypoxia-inducible factor 1-alpha otherwise known as HIF1 alpha (HIF1A) or ARNT interacting protein, a widely expressed bHLH-PAS transcription factor, which acts as a critical regulatory protein in the host response to hypoxia (Kubis et al. 2005).HIF1A and HIF1B, also known as ARNT form the highly-conserved heterodimeric HIF-1 transcriptional complex (Carmeliet et al. 1998). During hypoxic conditions, HIF1A accumulates in the nucleus and activates the transcription of many genes encoding proteins involved in the production of oxygen delivery and metabolic adaptation, such as those for vascular endothelial growth factor (VEGFA) . Under normal oxygen conditions, HIF1A is targeted by hypoxia inducible factor prolyl-hydroxylases, followed by rapid protease degradation, which is inhibited during hypoxia (Hirsilä et al. 2003). Overexpression of HIF1A occurs in many common human cancers, including pancreatic, bladder and renal carcinomas (Shibaji et al. 2003).Mouse anti Human HIF1a, clone Halpha111a has been successfully used for the identifcation of HIF1a in human samples by both immunohistochemistry and western blotting.

ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF-1-alpha, HIF1A, Hypoxia-inducible factor 1-alpha, Member of PAS protein 1, MOP1, PAS domain-containing protein 8, PASD8