Anti-HIF-1 alpha Antibody [ESEE122] (A305080)

Name: Anti-HIF-1 alpha Antibody [ESEE122]
Brand: Antibodies.com
SKU: A305080
Price: 450.0 USD
Availability: InStock

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$450

Mouse monoclonal [ESEE122] antibody to HIF-1 alpha for WB, IHC, ICC/IF and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

Cytoplasm Marker Mitochondrial Marker Nuclear Marker

Specifications

Name

Anti-HIF-1 alpha Antibody [ESEE122]

Description

Mouse monoclonal [ESEE122] antibody to HIF-1 alpha.

Specificity

This antibody is specific to HIF-1 alpha.

Applications

WB, IHC, ICC/IF, ELISA

Dilutions

WB: 1:1,000, IHC: 1:100, ICC/IF: 1:50

Reactivity

Human, Mouse, Rat, Bovine

Immunogen

Recombinant fragment corresponding to amino acids 329-532 of mouse HIF-1 alpha.

Host

Mouse

Clonality

Monoclonal

Clone ID

ESEE122

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~116 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF-1-alpha, HIF1A, Hypoxia-inducible factor 1-alpha, Member of PAS protein 1, MOP1, PAS domain-containing protein 8, PASD8

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-HIF-1 alpha Antibody [ESEE122] (A305080)

Western blot analysis of human Cervical cancer cell line (HeLa) lysate showing detection of HIF1 alpha protein using Anti-HIF-1 alpha Antibody [ESEE122] (A305080) at 1:500 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.

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Immunohistochemistry - Anti-HIF-1 alpha Antibody [ESEE122] (A305080)

Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-HIF-1 alpha Antibody [ESEE122] (A305080) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Membranous and cytoplasmic localization in the epidermis, positive hair follicles, muscle and dermis..

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