Anti-HIF-1 alpha Antibody (A295407)

Name: Anti-HIF-1 alpha Antibody
Brand: Antibodies.com
SKU: A295407
Price: 425.0 USD
Availability: InStock

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$425

Rabbit polyclonal antibody to HIF-1 alpha for WB, IP, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

Cytoplasm Marker Mitochondrial Marker Nuclear Marker

Specifications

Name

Anti-HIF-1 alpha Antibody

Description

Rabbit polyclonal antibody to HIF-1 alpha.

Applications

WB, IP, IHC, ICC/IF

Dilutions

ICC/IF: 1:100-1:500, IHC: 1:100-1:500, IP: 2-10 µg/1 mg lysate, WB: 1:2,000-1:10,000

Reactivity

Human, Mouse

Immunogen

Synthetic peptide within amino acids 775 and the C-terminus of human HIF-1 alpha.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Antigen affinity purification.

Concentration

200 µg/ml

Product Form

Liquid

Formulation

Supplied in Tris-Buffered Saline with 0.1% BSA and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

General Notes

This antibody was affinity purified using the immunising peptide immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1. 4 equals 1.0 mg of IgG.

Synonyms

ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF-1-alpha, HIF1A, Hypoxia-inducible factor 1-alpha, Member of PAS protein 1, MOP1, PAS domain-containing protein 8, PASD8

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Immunoprecipitation - Anti-HIF-1 alpha Antibody (A295407)

Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HepG2 cells that were either treated with 200 µM cobalt chloride or left untreated. Antibodies: Anti-HIF-1 alpha Antibody (A295407) was used for WB at 0.04 µg/ml and for IP at 6 µg/mg lysate. Detection: Chemiluminescence with an exposure time of 3 seconds.

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Western Blot - Anti-HIF-1 alpha Antibody (A295407)

Samples: Whole cell lysate (50 µg) from NIH3T3 cells that were either treated with 200 µM cobalt chloride (+) or mock treated (-). Antibodies: Anti-HIF-1 alpha Antibody (A295407) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with exposure times of 3 seconds. Lower Panel: Rabbit anti-COPB2 antibody.

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Western Blot - Anti-HIF-1 alpha Antibody (A295407)

Samples: Whole cell lysate (50 µg) from HepG2 cells that were either treated with 200 µM cobalt chloride (+) or mock treated (-). Antibodies: Anti-HIF-1 alpha Antibody (A295407) was used for WB at 0.04 µg/ml. Detection: Chemiluminescence with exposure times of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody.

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Immunocytochemistry - Anti-HIF-1 alpha Antibody (A295407)

Sample: Formaldehyde-fixed asynchronous HeLa cells treated with 200 µM cobalt chloride (left) or mock treatment (right). Antibody: Anti-HIF-1 alpha Antibody (A295407) Lot A (upper images) and Lot B (lower images) used at a dilution of 1:100 (2 µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated. Counterstain: DAPI (blue) and fluorescent phalloidin (green).