Anti-Hexokinase 1 Antibody [4D7] (A279371)

Anti-Hexokinase 1 Antibody [4D7]

Mouse monoclonal [4D7] antibody to Hexokinase 1.

This antibody recognises human hexokinase 1, a 102 kDa major allosteric enzyme of the glycolytic pathway, which catalyses the phosphorylation of glucose to form glucose-6-phosphate (Glc-6-P).Hexokinase 1 is the first of four distinct mammalian hexokinase isozymes (hexokinase type 1 to 4), expressed in the outer membrane of mitochondria and is regulated (inhibited) by its product Glc-6-P. A deficiency in hexokinase 1 is implicated in the rare hereditary autosomal recessive disease known as nonspherocytic haemolytic anaemia. recognises an epitope common to all isoforms of human hexokinase.

ELISA, WB

WB: 1:1,000 - 1:2,000

Human, Mouse

Recombinant human hexokinase 1 (amino acids 1-917) purified from E. coli.

MIAAQLLAYYFTELKDDQVKKIDKYLYAMRLSDETLIDIMTRFRKEMKNGLSRDFNPTATVKMLPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEKNQNVHMESEVYDTPENIVHGSGSQLFDHVAECLGDFMEKRKIKDKKLPVGFTFSFPCQQSKIDEAILITWTKRFKASGVEGADVVKLLNKAIKKRGDYDANIVAVVNDTVGTMMTCGYDDQHCEVGLIIGTGTNACYMEELRHIDLVEGDEGRMCINTEWGAFGDDGSLEDIRTEFDREIDRGSLNPGKQLFEKMVSGMYLGELVRLILVKMAKEGLLFEGRITPELLTRGKFNTSDVSAIEKNKEGLHNAKEILTRLGVEPSDDDCVSVQHVCTIVSFRSANLVAATLGAILNRLRDNKGTPRLRTTVGVDGSLYKTHPQYSRRFHKTLRRLVPDSDVRFLLSESGSGKGAAMVTAVAYRLAEQHRQIEETLAHFHLTKDMLLEVKKRMRAEMELGLRKQTHNNAVVKMLPSFVRRTPDGTENGDFLALDLGGTNFRVLLVKIRSGKKRTVEMHNKIYAIPIEIMQGTGEELFDHIVSCISDFLDYMGIKGPRMPLGFTFSFPCQQTSLDAGILITWTKGFKATDCVGHDVVTLLRDAIKRREEFDLDVVAVVNDTVGTMMTCAYEEPTCEVGLIVGTGSNACYMEEMKNVEMVEGDQGQMCINMEWGAFGDNGCLDDIRTHYDRLVDEYSLNAGKQRYEKMISGMYLGEIVRNILIDFTKKGFLFRGQISETLKTRGIFETKFLSQIESDRLALLQVRAILQQLGLNSTCDDSILVKTVCGVVSRRAAQLCGAGMAAVVDKIRENRGLDRLNVTVGVDGTLYKLHPHFSRIMHQTVKELSPKCNVSFLLSEDGSGKGAALITAVGVRLRTEASS

Mouse

Monoclonal

4D7

IgG2a

Protein G affinity chromatography of ascites.

1 mg/ml

Approximately 102 kDa in HepG2 cell lysates.

Liquid

Supplied in Phosphate Buffered Saline with 10% Glycerol and 0.02% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human hexokinase 1, clone 4D7 recognizes human hexokinase 1, a 102 kDa major allosteric enzyme of the glycolytic pathway, which catalyses the phosphorylation of glucose to form glucose-6-phosphate (Glc-6-P).Hexokinase 1 is the first of four distinct mammalian hexokinase isozymes (hexokinase type 1 to 4), expressed in the outer membrane of mitochondria and is regulated (inhibited) by its product Glc-6-P. A deficiency in hexokinase 1 is implicated in the rare hereditary autosomal recessive disease known as nonspherocytic haemolytic anaemia (Newman et al. 1980). Mouse anti hexokinase 1, clone 4D7 recognizes an epitope common to all isoforms of human hexokinase.

Brain form hexokinase, Hexokinase type I, Hexokinase-1, Hexokinase-A, HK I, HK1