Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
BHLHB39, Class B basic helix-loop-helix protein 39, Hairy and enhancer of split 1, Hairy homolog, Hairy-like protein, hHL, HL, HRY, Transcription factor HES-1
Western Blot - Anti-Hes1 Antibody [ARC0513] (A305844)
Western blot analysis of extracts of various cell lines, using Anti-Hes1 Antibody [ARC0513] (A305844) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Hes1 Antibody [ARC0513] (A305844) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Anti-Hes1 Antibody [ARC0513] (A305844) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Western Blot - Anti-Hes1 Antibody [ARC0513] (A305844)
Immunoprecipitation analysis of 600µg extracts of rat testis cells using 3µg of Anti-Hes1 Antibody [ARC0513] (A305844). This Western blot was performed on the immunoprecipitate using Anti-Hes1 Antibody [ARC0513] (A305844) at a dilution of 1:500.
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