This antibody recognises human hemoglobin, a ~66 kDa metalloprotein composed of four globular subunits each of which is associated with an iron-containing heme group. The heme group is involved with oxygen transport from the lungs to the tissues and the globin molecule plays a major role in transporting carbon dioxide in the reverse direction.Hemoglobin is found in the erythrocytes of all vertebrates. There is a wide diversity of amino acid sequences and substitutions within mammalian hemoglobins, however the molecular weight is generally around 66 kDa with an iron content of about 0.34%. The level of hemoglobin in the blood is used in the diagnosis of anemia; levels in the feces are indicative of various clinical conditions.Sheep anti Human Hemoglobin antibody shows <10% cross-reactivity with bovine hemoglobin.
Applications
WB, ELISA
Dilutions
WB: 1:100 - 1:1,000
Reactivity
Human
Immunogen
Hemoglobin purified from human erythrocytes.
Host
Sheep
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Concentration
5 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Sheep anti Human Hemoglobin antibody recognizes human hemoglobin, a ~66 kDa metalloprotein composed of four globular subunits each of which is associated with an iron-containing heme group. The heme group is involved with oxygen transport from the lungs to the tissues and the globin molecule plays a major role in transporting carbon dioxide in the reverse direction.Hemoglobin is found in the erythrocytes of all vertebrates. There is a wide diversity of amino acid sequences and substitutions within mammalian hemoglobins, however the molecular weight is generally around 66 kDa with an iron content of about 0.34%. The level of hemoglobin in the blood is used in the diagnosis of anemia; levels in the feces are indicative of various clinical conditions.Sheep anti Human Hemoglobin antibody shows <10% cross-reactivity with bovine hemoglobin.